Central Facilities
Fluorimeter
  • Fluorescence, Phosphorescence, Chemiluminescence and Bioluminescence Modes
  • All Reflective Optics with quartz over-coated optics. Schwarzchild Source Optics. Both provide for better sensitivity
  • High Sensitivity- Limit of detection
    • 1.0 picoMolar fluorescein using standard cell
    • 10 picoMolar fluorescein using 40 ul microcell
    • 750:1 S/N for Raman band of H2O, 350 nm excitation, excitation and emission slits 10 nm.
  • Instrument purging sample compartment windows & sample compartment
  • Xenon Flash Lamp only during acquisition. No continuous sources allowed
  • Lamp Pulse width at half peak height »2 us, peak power equivalent to 75 kW
  • Can acquire 80 data points/second for single cell kinetics
  • Fast scan speed of 24,000nm/min without wavelength shifts versus slower scan speeds
  • Limiting Resolution <1.5nm
  • Spectral band pass - Excitation: 1.5, 2.5, 5,10,20 nm & Emission: 1.5, 2.5, 5,10, 20 nm
  • Wavelength Reproducibility of +/-0.2nm
  • Wavelength Accuracy of +/-0.5nm at 541.2nm and +/- 1.0 nm from 200-900 nm
  • No degradation of photosensitive or heat-sensitive samples
  • Large Sample Compartment for future accessories and access (greater than 675 cubic inches, i.e.. Width x Depth x Height)
  • Room light immunity for most fluorescent samples
  • Separate monochromator for Excitation
  • Separate monochromator for Emission
  • Wavelength Range from 200-900nm for both excitation & emission monochromator; zero order selectable
  • Red Sensitive Photomultiplier tube is standard for sample path (prefer R928)
  • Reference path must have separate R928 Photomultiplier tube for detector
  • Able to run 40 microliters of sample volume in a microcuvette
  • Able to run <500 microliters of sample volume in a standard 10mm cuvette
  • Custom Development Language for user control of hardware & custom applications
  • Able to capture one phosphorescence data point every 1 microsecond up to 10 seconds
  • Able to capture one fluorescence data point every 12.5 milliseconds up to 999 sec.
  • Able to capture one bio/chemiluminescence data point every 40 microseconds up to 10 sec.
  • Able to e-mail data unattended right after run completed (PC must be connected to Internet)
  • Built-in excitation & emission filters to exclude secondary light
  • 384/96 well plate reader
  • Bio Software to include Thermal, Concentration, Simple/Advanced Reads, Validation, Kinetics applications
  • Internal Grams 3-D plotting software included
  • Detector Voltage settings 4 fixed & also completely manual
  • Repetitive scanning
    Maximum number of cycles -1000
    Maximum cycle time 1000 minutes

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