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Omair's Project Page - RISE summer 2005 |
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Intern: Omair Saadat, Electrical Engineering Department, Stanford University
Mentor: David K. Wood
Faculty Supervisor: Andrew N. Cleland
Department: Physics |
MICROFABRICATION OF A CELL-SORTING DEVICE
We fabricated a microfluidic device designed to sort cells based on the
cell’s impedance, which in turn depends on the amount of DNA present in the cell.
This device was composed of an antenna and microfluidic channels made of
poly-dimethyl-siloxane (PDMS). We fabricated a mold composed of a silicon substrate
and a thick photo-resist, SU-8 and used it to cast the PDMS devices.
A precise pressure pump was used to pump a silane solution with dissolved
polystyrene beads through the device. We used the polystyrene beads to simulate
actual cells. We used a high voltage switch to electro-osmotically switch the
flow of the fluid from one channel to the other. We intend to use this feature
to sort cells by direct the fluid to either one channel to another. We coupled
this switch to an antenna that could detect cells flowing through the fluid by
detecting impedance change caused by the cells.
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