Gel Permeation Chromatography (GPC) is a separation method for the determination of molecular weight averages (Mn) and molecular weight distribution (PDI=Mw/Mn). The testing report can include calibration plot, chromatogram, number average molecular weight and PDI of the polymer. GPC-chloroform is using chloroform as a solvent. Gel Permeation Chromatography (GPC) separates molecules on the basis of hydrodynamic size (not Mw). The large molecules move more rapidly through the GPC column, and in this way the mixture can be separated. The elution volume of a molecule is related to its hydrodynamic volume. To relate the elution volume to the molecular weight of a polymer, a calibration with molecular weight standards is needed.
Pump: Waters Alliance HPLC System, 2690 Separation Module.
Two columns: Agilent, PLgel, 5 µm MiniMIX-D, 250 x 4.6 mm + guard (MW linear range 200 - 400,000 g/mol)
Waters 2410 Differential Refractometer (RI)
Waters 2998 Photodiode Array Detector (PDA)
Solvent: Chloroform with 0.25% TEA
Flow Rate: 0.35 mL/min
Injection: 40 μL.
MRL room 1043
43 Maple Street
Milvord, MA 01757
Internet - http://www.waters.com
Instrument Information and/or Manual:
|GPC Chloroform Manual||125.42 KB|
Please contact Rachel Behrens (rachel [at] mrl [dot] ucsb [dot] edu) to schedule training. Before training starts please read the MANUAL.