Dynamic Light Scattering

DLS measures particle size distributions in solutions and suspensions.

Nanoparticles, biopolymers, environmental monitoring, macromolecules

Measurements

The DynaPro NanoStar™ from Wyatt Technology is Dynamic Light Scattering (DLS) instrument that is used for the analysis of protein solutions, aggregates, buffers, nanoparticles, polymers, or other products in solution. In a DLS measurement, the time-dependent fluctuations in the scattered light are measured by a fast photon counter. The fluctuations are directly related to the rate of diffusion of the molecule through the solvent. Therefore, the fluctuations can be analyzed to determine a hydrodynamic radius for the sample (0,5 nm to 1000 nm). Molar mass range: 300 Da to 10⁶ Da. Minimum concentration = 0,1 mg/ml.
 

Equipment
Instrument: DynaPro NanoStar™ from Wyatt Technology
Temperature: 22°C-150°C
Laser wavelength: 662 nm
Laser power: 100 mW

Cuvette: 1 µl (standard quartz-cuvette). Disposable cuvettes available (3 µl and 50 µl)
 

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