Isothermal Titration Calorimetry

The ITC measures heat flows during molecular interactions as liquid sample is injected and mixed into a cell.

Surfactants, macromolecules, pharmaceuticals, enzyme kinetics, binding affinity

Measurements

Nano ITC directly measures, via the differential power between the reference cell and the sample cell, heat evolved or absorbed in liquid samples as a result of titrating precise amounts of reactants. The data processing software can use fitting models to calculate reaction stoichiometry (n), binding constant (Kh), enthalpy (ΔH) and entropy (ΔS).

Equipment

Instrument: TA Instruments Nano ITC Microcalorimeter

Cell: Nano ITC gold cylindrical cell (1 mL)

Degassing Station: Includes stirring platform, vacuum chamber, vacuum port, temperature control and electronic timer.

Temperature: 15^oC - 80^oC

Titration Syringes: 100uL and 250 µL

Software:

ITCRun for data collection, NanoAnalyze for data analysis

Interested?

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