Photothermal mIRageLS OPTIR

The mIRage-LS optical photothermal infrared (O-PTIR) microscope is capable of simultaneous, sub-micron Infrared (IR) and Raman analysis and IR imaging. It breaks the diffraction limit of traditional micro-FTIR through the use of a ‘pump’ IR laser and a ‘probe’ green laser so that the resolution is determined by the spot size of the green laser, approximately 500 nm. Both IR and Raman spectra can be acquired simultaneously by selecting specific sample locations. 

While the mIRage is designed for sub-50 micron chemical characterization, the sample chamber can accommodate samples as large as one vertical inch. A wide variety of sample types (microplastics, multi-component polymer films, powders, biological cells, organic contaminants on semiconductors) are ideally suited for this technique. In addition, tissue can be spectroscopically mapped over large regions using single wavenumber mapping (fastest) or hyperspectral mapping where a full spectrum is acquired at each pixel. Standard histological sections are ideal for tissue/cell imaging. Unlike traditional IR microscopy, it is possible to use fresh tissue sections that are fully hydrated. As with all microscopies, flat surfaces are best to maintain a consistent focal plan, especially while imaging. While polarization experiments are not automated, they are possible by rotating the sample in the compartment. See some representative data and system specifications below. 

If you are interested in trying the OPTIR for a proof-of-concept experiment, please contact Amanda Strom (strom@ucsb.edu) and fill out the sample description form. Please consult TEMPO staff for detailed advice on sample preparation methods. The mIRage is open-access and training is available for any users of the MRL shared facilities. If you are not a user of MRL shared facilities, become one!

Internal recharge rate: $42/hr