Extracellular vesicles (EVs) are vesicles released by cells that carry protein, help with cell to cell communication, and other bioactive components. This study focuses on two different EV types: biofluid EVs which move freely in biofluids, and tissue EVs, which do not move freely but are often trapped inside the extracellular matrix (ECM). Tissue EVs are thought to get retained due to its ability to adhere to the ECM. The goal is to determine if the protein corona plays a role in EV adhesion to ECM. Binding of EVs will be modeled by encapsulating isolated EVs into collagen liquid hydrogels, mimicking tissue structure, and quantifying particle release over time. I hypothesize that the protein corona increases EV adhesion to the ECM. EVs will be analyzed by size, concentration and protein composition using Nanosight, SDS-PAGE, Western blot, and MicroBCA. Data will reveal whether the protein corona plays a significant role in ECM adhesion.